解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Kainic acid-induced seizures produced early (2 hr) generalized edema and later (24 and 48 hr) necrotic edema in temporal cortex and hippocampus as measured by specific gravity changes. Mannitol given during the seizure partially protected against the early edema and prevented the necrotic edema indicating early edema ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01000241
更新日期:1987-06-01 00:00:00
abstract::Exposure of rat pheochromocytoma PC12 cells to 0.1 mM 6-aminonicotinamide (6AN) for 24 hours resulted in a 500-fold increase in 6-phosphogluconate indicating active metabolism of glucose via the oxidative enzymes of the pentose phosphate pathway. Amounts of 6-phosphogluconate that accumulated in 6AN-treated cells at 2...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01000236
更新日期:1987-06-01 00:00:00
abstract::The activity of gamma-glutamyltransferase localized in isolated brain synaptic membranes- and microvessels-enriched fractions was assayed after treatment of rats with either phenobarbital or ethanol. Phenobarbital increased the activity of gamma-glutamyltransferase in microvessels, without alteration of synaptic membr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00993245
更新日期:1987-04-01 00:00:00
abstract::The effect of light exposure on the protein patterns of optic lobe and forebrain of the chick embryo was analysed by a high-resolution micro-two-dimensional polyacrylamide gel electrophoresis and computerized quantitation. Experiments were done on three groups of eggs: control group was incubated in the dark; simultan...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00993240
更新日期:1987-04-01 00:00:00
abstract::Chronic thiamine deprivation in the rat leads to ataxia, loss of righting reflex and neuropathological damage to lateral vestibular nucleus. Before onset of neurological symptoms, transketolase (TK) activities were found to be selectively reduced by 25% in lateral vestibular nucleus and surrounding pons. Further progr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00972141
更新日期:1987-03-01 00:00:00
abstract::The extracellular amino acid content was measured in the parietal cortex in portacaval and sham operated rats, using the brain dialysis technique. The amino acid content of the perfusate was determined for 10 min before and during stimulation with potassium chloride. Basal levels of aspartate, glutamine, glycine, meth...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00972136
更新日期:1987-03-01 00:00:00
abstract::Neurons, purified by bulk-isolation procedures from 10 day-old rat brain, are 80-90% homogeneous. Contaminants are primarily blood vessels and occasional oligodendroglia. All sizes and shapes of neurons are obtained, as they are isolated from all areas of the brain, except the cerebellum. Neurotransmitter enzyme activ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971368
更新日期:1987-01-01 00:00:00
abstract::The effect of phenobarbital (100 mg/kg i.p.) and 6-aminonicotinamide (6AN) (35 mg/kg i.p.) on enzyme activities related to energy transduction was investigated on the homogenate "in toto", non-synaptic mitochondrial fraction and synaptosomal fraction isolated from different rat brain areas (cerebral cortex, hippocampu...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971361
更新日期:1987-01-01 00:00:00
abstract::The anterograde axonal transport of choline-phosphoglycerides was studied in sciatic nerve motoneurons of adult (3-month-old) and aged (24-month-old) rats. After the spinal cord injection of [2-3H]glycerol, choline-phosphoglycerides; the major phospholipid class was transported along the nerve. The axonal transport ra...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971365
更新日期:1987-01-01 00:00:00
abstract::The effects of graded doses of D-pipecolic acid (0.005-5 micrograms/animals s.c.) on tolerance to the hypothermic effect of ethanol (4 g/kg i.p.) were investigated in mice. D-Pipecolic acid itself did not change the core temperature or the acute hypothermic response to a single dose of ethanol. Repeated D-pipecolic ac...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967746
更新日期:1986-12-01 00:00:00
abstract::The cellular uptake of the GABA-transaminase inhibitors gamma-vinyl GABA (GVG) and gamma-acetylenic GABA (GAG) was studied in cultured neurons and astrocytes. By the use of the individual enantiomers R- and S-GVG and R- and S-GAG it could be shown that in both cell types only the S-enantiomers could be actively transp...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965769
更新日期:1986-11-01 00:00:00
abstract::Postmortem changes in the activity of GDH, GAD, GABA-T, and GS in the adult forebrain and cerebellum and in the forebrain of newborns have been investigated. The activities of GDH, GAD, and GS were stable till 12 hr after death in both adult and young brain regions studied. The activity of GABA-T was stable till 30 mi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966226
更新日期:1986-10-01 00:00:00
abstract::The phospholipid requirement for Ca2+-stimulated, Mg2+-dependent ATP hydrolysis (Ca2+/Mg2+-ATPase) and Mg2+-stimulated ATP hydrolysis (Mg2+-ATPase) in rat brain synaptosomal membranes was studied employing partial delipidation of the membranes with phospholipase A2 (Hog pancreas), phospholipase C (Bacillus cereus) and...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966224
更新日期:1986-10-01 00:00:00
abstract::We have attempted to show eventual modifications in the brain protein synthesis apparatus of rat during the first three weeks after birth. Through this time we noted a steady decrease (about 60%) in the free polysomes, when expressed relative to tissue weight. This decrease does not correlate with changes in the polys...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966130
更新日期:1986-09-01 00:00:00
abstract::Populations of enriched glial precursor cells and astrocytes isolated from primary cultures of newborn rat brain were used to study the synthesis of sulfated glycoproteins. Both cell types incorporated [3H]glucosamine and [35S]sulfate into carbohydrate side chains of proteoglycans and glycoproteins. The rate of incorp...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965947
更新日期:1986-08-01 00:00:00
abstract::When excised goldfish spinal cord is kept in physiological saline at room temperature, the myelin multilayers swell. As studied by X-ray diffraction, the original repeating distance of 150 A (AS myelin) swells to 177 A (AL myelin); no intermediate distances are seen. At least 70% of the myelin can undergo this gradual...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965941
更新日期:1986-08-01 00:00:00
abstract::Cathepsin B (EC 3.4.22.1) was purified 746-fold with a 21% recovery from bovine brain by autolysis, fractional precipitation with acetone, carboxy-methyl-Sephadex chromatography, affinity chromatography on a cystamine containing column and gel filtration chromatography. The purified cathepsin B eluted on gel filtratio...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965209
更新日期:1986-06-01 00:00:00
abstract::The binding of the enkephalin dimer [D-Ala2, Leu5-NH-CH2-]2 (DPE2) is characterized by its high affinity for receptors on NG108-15 hybrid cells, the affinity constant K = 4.7 X 10(9) M-1 is up to 8-fold that of monomers (0.6 to 1.0 X 10(9) M-1), and a maximal binding capacity equal to one half that of the monomers. Ki...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965208
更新日期:1986-06-01 00:00:00
abstract::Incomplete ischemia of the spinal cord was produced in dogs by 40 min occlusion of the abdominal aorta that was followed by 5-40 min of recirculation. Amino acid incorporation into ribosomes in vitro in the presence of venous blood sera was estimated. The most significant reduction in incorporation was produced by ser...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965210
更新日期:1986-06-01 00:00:00
abstract::Somatostatin contents of striatum, hippocampus, and pons medulla have been followed in two inbred strains of mice (C57B1/6J and Balb/cJ) with aging and long term alcohol exposure (over a 25 month period of intoxication). Marked strain dependent differences in basic levels of somatostatin and genotypic variations in re...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965332
更新日期:1986-05-01 00:00:00
abstract::Levels of free amino acids were determined quantitatively in whole ocular tissues of the rat eye with aid of a sensitive amino acid analyzer. The tissues studied were the retina, vitreous, lens, iris-ciliary body, and cornea. The retina and lens contained a more concentrated free amino acid pool than other tissues. Th...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965323
更新日期:1986-04-01 00:00:00
abstract::Suspensions of rat brain microsomes, synaptosomes, and synaptic vesicles were able to convert adenosine to inosine by means of adenosine deaminase. Isosbestic points of this transformation, at 222, 250 and 281 nm, remained unchanged with time-course. This fact suggests that adenosine deaminase (ADA, E.C. 3.5.4.4) is l...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965316
更新日期:1986-04-01 00:00:00
abstract::The subcellular distribution of Ca2+/calmodulin-stimulated adenylate cyclase activity was studied in comparison with that of guanine nucleotide-stimulated cyclase activity. The distributions of these activities were similar among the crude fractions but differed among the purified subsynaptosomal fractions. The specif...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965018
更新日期:1986-03-01 00:00:00
abstract::The activity of guanine deaminase (GAH, E.C.3.5.4.3) was lower in rat cerebellum soluble and microsomal fractions than in rat brain subfractions. Adenosine deaminase (ADA, E.C.3.5.4.4) activity was released in higher proportion than guanine deaminase, purine nucleoside phosphorylase (PNP, E.C.2.1.2.4), 5'-nucleotidase...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965016
更新日期:1986-03-01 00:00:00
abstract::The kinetics and specificity of taurine and beta-alanine uptake were studied in primary cultures of rat astrocytes under identical experimental conditions. The uptake consisted of nonsaturable penetration and saturable high-affinity transport that was strictly sodium dependent. The cells accumulated taurine more effec...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967969
更新日期:1986-02-01 00:00:00
abstract::Rats were long-term morphine-intoxicated by a fluid-diet model ensuring an equal nutrient intake in morphinized and control rats. Uptake of neurotransmitters and D-ala2-met5-enkephalinamide (Enk) was studied in the particulate fractions (obtained at 10,000 g) from well defined brain regions of long-term intoxicated an...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967978
更新日期:1986-02-01 00:00:00
abstract::The uptake of radioactive ethanolamine has been studied in exclusively neuronal and glial cell cultures from dissociated cerebral hemispheres of chick embryos. Both cell types show saturable kinetics; neurons have an apparent Km of 6.7 microM, Vmax 41.4 pmol mg prot.-1 min-1 and glial cells a Km of 119.6 microM, Vmax ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965162
更新日期:1986-01-01 00:00:00
abstract::Mouse isolated vas deferens preincubated with [3-H]noradrenaline was superfused and the effect of alpha 1-adrenoceptor agonists was studied on the release of total radioactivity ([3H]noradrenaline + 3H-metabolites) and [3H]noradrenaline. Reverse phase high pressure liquid chromatography (HPLC) combined with scintillat...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965167
更新日期:1986-01-01 00:00:00
abstract::Discrete populations of anti-S49 antibodies were found in the antisera of Lewis rats recovered from S49-induced experimental allergic encephalomyelitis (EAE). A potent inducer of EAE in Lewis rats, S49 is a synthetic peptide representing residues 69-84 of bovine myelin basic protein but with deletions at Gly-77 and Hi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00988601
更新日期:1985-12-01 00:00:00
abstract::In this report data are summarized on changes in the quantity of proteolipid protein (PLP), its amino acid composition, and the lipid moiety of these lipid-protein complexes in rat brain during postnatal development. In all three parts of the central nervous system (CNS) studied (cerebral hemispheres, medulla oblongat...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract::The present work describes the kinetics of enkephalin hydrolysis by plasma enzymes and the fragmentation pattern of both the parent peptide and of the first hydrolysis by-products. The degradation kinetics were followed by positive identification of the hydrolysis fragments by chromatographic methods, by amino acid an...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964980
更新日期:1985-10-01 00:00:00
abstract::In an effort to develop an animal model of hyperpipecolatemia, the uptake of pipecolic acid (PA) in the brain and changes of PA levels in serum following administration of D,L-PA were studied in the mouse using a new sensitive HPLC-EC method. Following i.p. injections (250 mg/kg) to adult male mice, the brain concentr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964981
更新日期:1985-10-01 00:00:00
abstract::Cloned fragments of members of the Drosophila and mouse major heat shock (hsp70) gene family were used to demonstrate that homologous sequences are present in the rabbit genome. After a physiologically relevant increase in body temperature of 3 degrees C, transcription of inducible hsp70 genes is detected in both the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964846
更新日期:1985-09-01 00:00:00
abstract::Radioactive azide derivatives of tetrodotoxin (TTX) were synthesized using 2-nitro-4-azidephenyl-[3H]beta alanine for the purpose of photolabeling of the Na channel. Three azide derivatives, N1, N2 and N3, were separated by ion exchange chromatography on Bio-Rex 70 resin and reversed phase high performance liquid chro...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965886
更新日期:1985-08-01 00:00:00
abstract::The calcium-activated phosphodiesteratic hydrolysis of 32P-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate in prelabeled nerve ending membranes is inhibited by the aminoglycosides neomycin and gentamicin, and to a lesser extent, by streptomycin. The inhibition is overcome by increasi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965878
更新日期:1985-08-01 00:00:00
abstract::Amphiphile dependency, solubility in aqueous solutions, and sensitivity to proteolysis of acetylcholinesterase (AChE) and nonspecific cholinesterase (nsChE) in the rat superior cervical ganglion were studied and compared to properties of soluble plasma cholinesterases. Ganglion AChE shows strong amphiphile dependency:...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964537
更新日期:1985-06-01 00:00:00
abstract::A panel of mouse monoclonal antibodies to rat and human myelin-associated glycoprotein (MAG) was developed. Normal mice were unresponsive to rat MAG, and successful immunization with rat MAG was only achieved in autoimmune NZB mice. By contrast, all strains of mice were responsive to human MAG. The monoclonal antibodi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964654
更新日期:1985-04-01 00:00:00
abstract::A double ligand enzyme-linked immunosorbent assay (ELISA) has been developed to detect antibodies against brain tissue antigens in the sera of patients with neurological diseases. The sera were tested on human white matter homogenate. The technique consists of successive incubations with the human serum to be tested, ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964651
更新日期:1985-04-01 00:00:00
abstract::Postnatal developmental patterns of activity for acid and alkaline deoxyribonuclease (DNase) were determined in the cerebellum of the androgenized female rat. Littermates were injected s.c. with either 1.25 mg testosterone propionate (TP) or vehicle 48 hours after birth. The enzymes and DNA content were measured in th...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964659
更新日期:1985-04-01 00:00:00
abstract::Release of endogenous dopamine (DA) and gamma-aminobutyric acid (GABA) from superfused rat caudate synaptosomes was monitored with liquid chromatography with electrochemical detection. Dopamine was analyzed by oxidative detection following alumina extraction while GABA was analyzed with reductive detection following p...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964602
更新日期:1985-03-01 00:00:00